3) At the bottom of the strip, click on "Show. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. SpectraMax® Microplate Readers and SoftMax® Pro Software. com Setup for Omnia® assays on SpectraMax®. both cuvette-based and microplate. Additionally, all BMG LABTECH microplate readers offer the possibility to measure in very small volumes, down to 2 µl of the sample by using the LVis Plate. From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Reads various kinds of 96-wells plates, and is equipped with shaking function. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. type: Clear: Add to Cart. The assay is carried out in a standard 96-well plate. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. SpectraMax Glo Steady-Luc Reporter Assay Kit. 5%in96-and384-wellmicroplates. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The instrument will read the plate, then the relative fluorescence units (RFUs) will be displayed in the Plate section of SoftMax Pro Software. Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. 9000-0756. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Avoid losing high signal. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Calcein AM is a widely used live-cell marker. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. Shop now. It is typically used to determine the concentration of nucleic acids and proteins. Enables accurate fluorescence data with every run. For example, microplates containing different samp les can be read using the same orThe plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Fluorescence measurements no longer require you to adjust the gain. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 314 low lamp level warning. Plate chamber design of the SpectraMax category 1 microplate readers. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). The fluorometric. Offer Ends In: 40 Days 05: 02: 03. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Offer Ends In: 45 Days 06: 20: 11. Application: For molecular biology testing. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 00. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. 0 out of 5 $ 2,999-20%. When displaced, changes in fluorescence can be read in an FP, FI, or TR-FRET depending on the assay format. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Category 2 and 3 microplate readers use gradient heating as well. Plate reader set to 50 reads. For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. SpectraMax M2 and SpectraMax M2e from Molecular Devices are multi- detection microplate readers with dual-monochromators, dual-mode cuvette ports and top- and bottom-reading capability (top-reading only on SpectraMax M2). Other features and benefits include: Eliminate changing out filters. NOTE: This assay differs from the previous one in that the fluorescent dye ThT or ThS is included in the aggregation reaction. Molecular devices SpectraMax M3 Pdf User Manuals. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemFAST AND ACCURATE METERTECH Microplate Reader $3,650. Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. 0 × 107) RLU 0. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. Additionally, by issuing a single read command, combined absorbance and fluorescence intensity assays can be run. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The spectrophotometric performance of the SpectraMax M2 and SpectraMax M2e is similar to the SpectraMax Plus, a dedicated absorbance plate reader. Temperature variation ± 0. Add to Cart. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Multimode Reader Comparison Chart. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. S. Fluorescence Photometric Performance (M2) Dual monochromators: 1 nm increments: EX: 250-850 nm: EM: 250-850 nm: Bandwidth (EX, EM). Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax Quant dsDNA Assay Kits. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The GloMax® line includes high-performance plate readers with a range of detection options. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive. Plate Readers. Microplate Readers. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Description 1. 5%in96-and384-wellmicroplates. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The excitation and the emission. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Tecan’s. View online or download Molecular devices SpectraMax M5 User Manual. In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. The flexibility of a high end multimode plate reader with environmental control. Sale price $9,995. Highly selective for dsDNA over ssDNA or RNA. Type: Microplate Reader. The IC50s by cell rounding and LysoTracker assays were 0. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). The SpectraMax M2 reader can be easily converted and optimized for very low-throughput to medium-high throughput analysis, faster, more accurate results and saving reagents. The SPECTRAmax® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits. It is particularly powerful when monitoring fast kinetic assays in a high. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. When connecting for the first time to either the SpectraMax L reader or StakMax system. Fig 2: Enzymatic response of synthetic compounds. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Tecan Infinite F200 Fluorescence Microplate Reader. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. We use Spectramax M5 microplate reader in our lab. Cells in each image are identified by SoftMax Pro Software and cell-by-cellThe Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Microscopes and Microplate Instrumentation. Align the two slots on the bottle holder with the two pegs on the rail. Featuring temperature control up toThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Published Dec 16, 2020 | Updated Dec 16, 2020. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. On the SpectraMax i3x reader, HTRF (cartridge) should be selected as the Optical Configuration. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. 3: The cuvette chamber. Select the Driver tab and click Update Driver. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. LuminescencePhotometricPerformance. Two important settings to obtain the best results in TRF assays. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 1. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. Figure 2-23: Bottle holder To install the bottle holder: 1. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Downloads. The purple holder is used for reading non-transparent plates from the top (e. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. These robust readers have been placed in labs from Antarctica to the. Biotek Elx800 Microplate Reader. (30. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. 0 out of 5 $ 3,895. Plate reader instructions Computer. Reader Documentation Version No. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Step 5: Click the software’s Read button. 7. I understand the OD measurement in a plate reader varies with the volume of the sample measured. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Microplate Reader Shelf. Multimode Readers Comparison Chart. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax Paradigm Detection Cartridge design utilizes an energy intensity optimization technique that allows the user to read up to seven logs of sample concentration in a single pass of a plate without any manual intervention. Dose-dependent enzyme essayVisualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Note: Check the Applies to section at the bottom of the page to make this is the proper driver for your plate reader. However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. SpectraMax M2 plate reader (Molecular Devices) was employed to measure the absorbance at 490 nm and 680 nm. 5,112,134 5,766,875. Three kits covering a wide range of dsDNA concentrations. 5). The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. . . SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Plate Shaking. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. 40266 Email: drugdiscoverytech@lifetech. The EarlyTox Live/Dead Assay Kit contains two markers for live or dead cells that are suitable for use with mammalian cells. With exceptional signal-to-noise ratio and a lower detection limit. Figure 2. Analyze the dataHere we report the use of the EarlyTox ™ Live/Dead Assay Kit in combination with SpectraMax ® fluorescence microplate readers (Figure 1). Five preferred detection modes. 25 to 3. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein. Fixes: General:SpectraMax M2 and M2e Multi-Mode. It allows you to explore cellular pathways and protein activation and expression in one system. 1 fmol/well 384-well plate) Luminescence Specifications Wavelength Range 300 to 700 nm Dynamic Range < 6 decades Sensitivity 10 amol ATP typical (flash) Fluorescence Polarization Specifications Light Source. When reading optical density at wavelengths below 340 nm, special UVtransparent,. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. Usage/Application: Lab. Listing: #4772697. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. For example, microplates containing different samp les can be read using the same or The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Category 2 and 3 microplate readers use gradient heating as well. An assay plate containing a 15-point standard curve was read on the SpectraMax. 0 × 103 to 2. assays, we can offer a microplate reader solution to help meet the. 1) Select the correct plate and the wells that you want to measure. Fluorescence measurements were acquired at 10 min intervals (ex: 485 nm and em: 535 nm) in a SpectraMax M2 fluorescence plate reader (Molecular Devices) operated in the 384-well microplate format. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Used. The SpectraMax 190 reader can be integrated with Molecular . Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. If you are going to read it from the bottom, you should use a clear-bottom black plate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. The pre-configured protocols can be selected for each assay and. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. SpectraMax M2, M2e;. To schedule a training session please contact Ewa Folta-Stogniew. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Add to Cart. Maximum signal with lowest background. . Add to Cart. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. 305 reference level saturation. Read the microplate Step 1: Place the microplate in the reader. 1 New Reader Support 1. SpectraMax M2 and use thereof is covered by issued (U. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). 00 CAD. ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Dose-dependent enzyme essay Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. I have been using the Molecular Devices SPECTRAmax GEMINI XS for the last 6 months, mostly in time-resolved fluorescence,. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. 9000-0756. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. 3) At the bottom of the strip, click on "Show. The systems have. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Basic "FL Plate Reader start up protocol" (print and bring along for the. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 0 out of 5 $ 9,995. If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before. (a) Chemical structures of 1 (HADP) and its analogues 2–4. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). We would like to show you a description here but the site won’t allow us. Luminex Magpix. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Download Application Note Plate reader settings for NanoOrange assay. The entry should be SpectraMax GEMINI EM. Data from The SpectraMax® iD3 Multi Mode Microplate . Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 10. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. 1 SoftMax Pro v4. okyo M LLC O T rinted in in rinted P. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceSeller assumes all responsibility for this listing. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. 58% for the detection module and 2. luminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. 00. Allows you to choose your sample format depending on your unique research and throughput needs. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. The chamber contains. The modular platform allows upgrading to expand functionality. Similar settings will work for other SpectraMax readers with fluorescence detection. A decrease in fluorescence with increasing staurosporine concentration was detected with the SpectraMax iD3 reader. Plate reader set to 50 reads. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Products : SpectraMax M2/M2e reader, SpectraMax. NanoQuant plate. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 7. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. the reader’s stated range. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. olecular saka. On the SpectraMax iD5 reader, install the 320/100 excitation filter and 616/10 and 665/10 emission filters, and then select these filters from the Excitation and Emission dropdown wavelength menus. Features. Important things to consider when purchasing a multimode microplate reader. Qualify the fluorescence performance of the SpectraMax . BMG Labtech - THERMOstar. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. spr SpectraMax iD5 SpectraTest ABS2 v1. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and. Plate Formats: 6, 12, 24, 48, 96, 384 wells: Light Source: Xenon Flash Lamp (1 joule/flash)The SpectraMax® iD3 Multi Mode Microplate . SpectraMax Spectrofluorometers. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. td. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. This reader's dual monochromators for. Devices’ StakMax® Microplate Stacker in a matter of minutes and . Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. An assay plate containing a 15-point standard curve was read on the SpectraMax. 1. Condition: Used - Good. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). plate over and over again. Add to Cart. KEY FEATURES • Use the touchscreen to easily set up methods, runSpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. 1. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Because. Model Description. Three data types for FP read types are also being introduced with v4. Conclusions. Optimized for fluorescence microplate readers. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). DNA quantification with fluorescence dyes. The Spectr. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. com 10. Temperature control range from Ambient +4º to 50ºC. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. LVF Monochromator™ : Excitation and Emission. A 96-well plate scheme was selected for the measurement mode and the wells selected for targeting the respective colonies arrayed. Microplate Readers. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Traditional microplate readers perform mostly biochemical assays—measuring how much light passes through or is emitted by a sample. The dual monochromator optics allow the. The password is. 7°C (gradient heating design). In the lower half of the window, left side, there is a box labeled reader. The M4 Reader offers the same modes as the M3 Reader, plus time-resolved fluorescence, and can be upgraded to include fluorescence polarization. Figure 6: Transcreener ADP2 FI assay principle. fluorescence intensity (Figure 6). Many of the following instruments are multimode readers that, in addition to luminescence detection, can also be used to measure UV. On microplate readers,. 8: theMicroplate Reader & Luminometer Selection Guide. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. Microplate Readers. View online or download Molecular devices SpectraMax M3 User Manual. A . 5 pM typical (0. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 0 out of 5. The SpectraMax® ABS Plus Microplate Reader can run . Bottom Monochromator: Fluorescein 2. CO 2 & O 2 control. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) that reduces background noise for excellent sensitivity and a wide dynamic range. Molecular Devices SpectraMax M2. STAKMAX. Optimized Settings to Validate the Molecular Devices SpectraMax® M2/M2e Microplate Readers with the Transcreener® Fluorescence Intensity Assays. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. 2. 5,112,134 5,766,875. A solution for low volume applications. 020 OD 2. 303 unable to cal dark current. made for this assay plate. Automated live cell imaging – cell counting and confluence. Explore how the Gemini EM Microplate Reader is a flexible spectrofluorometer capable of running many fluorescence-based assays using its dual. SpectraMax® M2/M2e Multimode Microplate Readers, with SoftMax® Pro Software, Molecular Devices.